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Step-by-Step Tutorial
PRACTICAL WALKTHROUGH · PARAMETERS · COMMON MISTAKES
Microfluidics enables precise control of fluid flow at the micrometer scale to produce uniform protein emulsions, high-shear homogenization, and enzyme encapsulation.
Origin: American (MIT) (1990s-2000s) — United States
See full technique reference →By manipulating flow rates (1–10 µL min⁻¹) and temperatures (25–37 °C), microfluidic devices generate droplets 10–100 µm in diameter, allowing proteins to remain intact while achieving high shear (>10⁵ s⁻¹) during homogenization. Encapsulation within lipid droplets preserves enzyme activity at 4 °C for over 30 days, with efficiencies exceeding 90 % at 25 °C.
Primary Reaction
Emulsification and shear-induced homogenization of proteins
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Key Variables