Growth and Maintenance of <i>Escherichia coli</i> Laboratory Strains

Abstract

Escherichia coli is a Gram-negative bacterium, commonly used in both teaching and research laboratories. This article includes protocols for the growth and maintenance of E. coli in any teaching- or research-associated laboratory. © 2021 Wiley Periodicals LLC. Basic Protocol 1: Growth of E. coli from frozen stocks Basic Protocol 2: Growth of E. coli in liquid media Basic Protocol 3: Enumeration of E. coli on solid media Basic Protocol 4: Storage of E. coli frozen stocks in glycerol Basic Protocol 5: Storage of E. coli in agar stabs Basic Protocol 6: Growth curve of E. coli liquid culture.