Functional Annotation Genome Unravels Potential Probiotic Bacillus velezensis Strain KMU01 from Traditional Korean Fermented Kimchi
Sojeong Heo, Jong‐Hoon Kim, Mi‐Sun Kwak, Moon‐Hee Sung, Do‐Won Jeong
Foods
Abstract
<i>Bacillus velezensis</i> strain KMU01 showing γ-glutamyltransferase activity as a probiotic candidate was isolated from kimchi. However, the genetic information on strain KMU01 was not clear. Therefore, the current investigation was undertaken to prove the probiotic traits of <i>B. velezensis</i> strain KMU01 through genomic analysis. Genomic analysis revealed that strain KMU01 did not encode enterotoxin genes and acquired antibiotic resistance genes. Strain KMU01 genome possessed survivability traits under extreme conditions such as in the presence of gastric acid, as well as several probiotic traits such as intestinal epithelium adhesion and the production of thiamine and essential amino acids. Potential genes for human health enhancement such as those for γ-glutamyltransferase, nattokinase, and bacteriocin production were also identified in the genome. As a starter candidate for food fermentation, the genome of KMU01 encoded for protease, amylase, and lipase genes. The complete genomic sequence of KMU01 will contribute to our understanding of the genetic basis of probiotic properties and allow for the assessment of the effectiveness of this strain as a starter or probiotic for use in the food industry.
Extracted Claims
5 claims extracted from this paper into the knowledge graph
Bacillus velezensis strain KMU01 encode survivability traits under extreme conditions
“Strain KMU01 genome possessed survivability traits under extreme conditions such as in the presence of gastric acid.”
Bacillus velezensis strain KMU01 encode γ-glutamyltransferase, nattokinase, and bacteriocin production
“Potential genes for human health enhancement such as those for γ-glutamyltransferase, nattokinase, and bacteriocin production were also identified in the genome.”
Bacillus velezensis strain KMU01 encode intestinal epithelium adhesion
“Strain KMU01 genome possessed survivability traits under extreme conditions such as in the presence of gastric acid, as well as several probiotic traits such as intestinal epithelium adhesion.”