Encapsulation of Lactobacillus in Low-Methoxyl Pectin-Based Microcapsules Stimulates Biofilm Formation: Enhanced Resistances to Heat Shock and Simulated Gastrointestinal Digestion
Caian He, Imca Sampers, Davy Van de Walle, Koen Dewettinck, Katleen Raes
Journal of Agricultural and Food Chemistry
Abstract
Encapsulation is a common approach to improve the bacterial survival of probiotics. In this study, two new low-methoxyl pectins (CMP-6 and CMP-8) were used as coating materials to produce microcapsules (MCs) for the encapsulation of <i>Lactobacillus acidophilus</i> LMG9433<sup>T</sup>, <i>Lactobacillus casei</i> LMG6904<sup>T</sup>, and <i>Lactobacillus rhamnosus</i> LMG25859. A fermentation test showed that encapsulation did not influence the fermentation ability of lactobacilli. The biofilm formation of encapsulated lactobacilli was stimulated when an <i>in situ</i> cultivation was conducted on MCs, which was verified by cryo-SEM observation. The resultant biofilm-forming MCs (BMCs) contained high-density bacterial cells (∼10<sup>10</sup> CFU/mL). Compared to planktonic lactobacilli, pectin-based MCs showed significant protection for encapsulated lactobacilli from heat shock and simulated gastric digestion. Especially, benefiting from the biofilm formation, BMCs provided higher protection with enhanced resistance to heat shock, freeze-drying, and gastrointestinal digestion than MCs. Our result highlighted the superior bacterial resistances of biofilm-forming probiotics encapsulated in pectinate microcapsules.
Extracted Claims
6 claims extracted from this paper into the knowledge graph
pectin-based microcapsules (MCs) show significant protection for encapsulated lactobacilli from heat shock and simulated gastric digestion
“Compared to planktonic lactobacilli, pectin-based MCs showed significant protection for encapsulated lactobacilli from heat shock and simulated gastric digestion.”
low-methoxyl pectins (CMP-6 and CMP-8) are used as coating materials to produce microcapsules (MCs) for the encapsulation of Lactobacillus acidophilus, Lactobacillus casei, and Lactobacillus rhamnosus
“In this study, two new low-methoxyl pectins (CMP-6 and CMP-8) were used as coating materials to produce microcapsules (MCs) for the encapsulation of <i>Lactobacillus acidophilus</i> LMG9433<sup>T</sup...”
biofilm-forming microcapsules (BMCs) provide higher protection with enhanced resistance to heat shock, freeze-drying, and gastrointestinal digestion
“Especially, benefiting from the biofilm formation, BMCs provided higher protection with enhanced resistance to heat shock, freeze-drying, and gastrointestinal digestion than MCs.”