Isolation and Gelling Properties of Duckweed Protein Concentrate
Maaike Nieuwland, Peter Geerdink, Nicole Engelen-Smit, I.M. van der Meer, Antoine H. P. America, Jurriaan J. Mes +3 more
ACS Food Science & Technology
Abstract
An isolation procedure for proteins from duckweed was optimized based on a previously developed method for protein isolation from sugar beet leaves. Optimization included the protocol for disrupting cells and protein recovery. With the optimized protocol, protein was isolated (protein yield 14.2%, RuBisCO yield 27%). The concentrate was off-white and contained 67.2% protein. The isolation procedure resulted in a large enrichment in RuBisCO (from 48% to 92%). Denaturation of duckweed protein concentrate was observed at 62 °C at pH 7, while heating at pH 4 did not show denaturation peaks. Solubility was good far from the iso-electric point and showed a minimum around pH 5. Gelling was better at pH 7 than at pH 4. At pH 7, duckweed gels were much stronger than soy and only slightly weaker compared to egg white protein, while at pH 4 duckweed gel strength was similar to soy and lower than egg white.
Extracted Claims
9 claims extracted from this paper into the knowledge graph
duckweed protein concentrate denatures at 62 °C at pH 7
“Denaturation of duckweed protein concentrate was observed at 62 °C at pH 7”
duckweed protein concentrate gels better at pH 7 than at pH 4
“Gelling was better at pH 7 than at pH 4”
duckweed protein concentrate has lower gel strength than egg white at pH 4
“At pH 4 duckweed gel strength was similar to soy and lower than egg white”