Self-Assembled Pea Protein Isolate Nanoparticles with Various Sizes: Explore the Formation Mechanism
Xiaolong Li, Qiutao Xie, Wenjie Liu, Baocai Xu, Bao Zhang
Journal of Agricultural and Food Chemistry
Abstract
Pea protein isolate nanoparticles (PPINs) were successfully prepared by potassium metabisulfite (K<sub>2</sub>S<sub>2</sub>O<sub>5</sub>). The disulfide bonds were disrupted by K<sub>2</sub>S<sub>2</sub>O<sub>5</sub>, and then the PPINs were formed through self-assembly. The average diameter of PPINs increased from 124.7 to 297.5 nm as the concentration of K<sub>2</sub>S<sub>2</sub>O<sub>5</sub> was increased from 2 to 8 mM, and the PPINs showed higher ζ-potentials (-32.2 to -35.8 mV) and unimodal distribution. The content of free sulfhydryl groups first increased and then decreased with the fracture and reformation of disulfide bonds. Subsequently, the increase of the β-sheet, which has considerable hydrophobicity, promoted the formation of PPINs. The formation mechanism of PPINs was explored by dissociation tests: hydrophobic interactions maintained the basic skeleton of PPINs, disulfide bonds stabilized the internal structure, and hydrogen bonds existed on the exterior of the particles. This study provided a simple and economical method to fabricate nanoparticles.
Extracted Claims
6 claims extracted from this paper into the knowledge graph
Formation mechanism of PPINs explored by dissociation tests
“The formation mechanism of PPINs was explored by dissociation tests: hydrophobic interactions maintained the basic skeleton of PPINs, disulfide bonds stabilized the internal structure, and hydrogen bo...”
Average diameter of PPINs increased from 124.7 to 297.5 nm
“The average diameter of PPINs increased from 124.7 to 297.5 nm as the concentration of K<sub>2</sub>S<sub>2</sub>O<sub>5</sub> was increased from 2 to 8 mM”
ζ-potentials of PPINs showed higher ζ-potentials (-32.2 to -35.8 mV)
“The PPINs showed higher ζ-potentials (-32.2 to -35.8 mV) and unimodal distribution.”