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Investigating the Probiotic Properties and Antimicrobial Activity of Lactic Acid Bacteria Isolated from an Iranian Fermented Dairy Product, Kashk — Foodgeist Research

2022peer-reviewed33 citationsOpen Access

Investigating the Probiotic Properties and Antimicrobial Activity of Lactic Acid Bacteria Isolated from an Iranian Fermented Dairy Product, Kashk

Bahareh Saboori, Fakhri Shahidi, Sara Hedayati, Ali Javadmanesh

Foods

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Abstract

In the present study, kashk samples were collected from two regions of Iran, the Fars (Abadeh) and Razavi Khorasan (Kalat) provinces. Fifteen bacteria were isolated and physiological and biochemical assays were performed. After identification to the genus level, eight isolates were identified as lactic acid bacteria (LAB) and subjected to molecular identification and probiotic properties assays. The results revealed that the isolates were <i>Enterococcus faecium</i> KKP 3772 (KF1), <i>Enterococcus faecium</i> C1 (KF2), <i>Pediococcus pentosaceus</i> H11 (KF3), <i>Pediococcus pentosaceus</i> VNK-1 (KK4), <i>Lactococcus lactis</i> RSg (KK1), <i>Enterococcus faecalis</i> P190052 (KK2), <i>Enterococcus mundtii</i> CECT972T (KK3), and <i>Lactiplantibacillus plantarum</i> PM411 (KK5). Only the numbers of <i>L. lactis</i> RSg (KK1) and <i>Lpb. Plantarum</i> PM411 (KK5) decreased to below 9 Log CFU/mL after acidic conditions (pH = 3) and showed weak antibacterial activity. <i>Enterococcus mundtii</i> CECT972T (KK3) and <i>E. faecium</i> C1(KF2) were highly susceptible to bile salts, while <i>P. pentosaceus</i> VNK-1 (KK4) and <i>P. pentosaceus</i> H11 (KF3) showed the highest resistance. All of the isolates were resistant to tetracycline and sensitive to chloramphenicol and gentamicin. The antimicrobial activity of <i>P. pentosaceus</i> VNK-1 (KK4) and <i>P. pentosaceus</i> H11 (KF3) was higher than other isolates and consequently, their inhibition zones were larger. The adhesion capabilities of LAB isolates to intestinal epithelial cells were evaluated by examining the auto-aggregation factor and cell surface hydrophobicity. The highest and lowest cell surface hydrophobicity and auto-aggregation were obtained from <i>P. pentosaceus</i> VNK-1 (KK4) and <i>E. mundtii</i> CECT972T (KK3), respectively. In general, <i>P. pentosaceus</i> VNK-1 (KK4) and <i>P. pentosaceus</i> H11 (KF3) have shown better probiotic properties as compared to other isolates.

Extracted Claims

11 claims extracted from this paper into the knowledge graph

compound_effect100% confidence

Pediococcus pentosaceus H11 (KF3) resistant bile salts

“Enterococcus mundtii CECT972T (KK3) and E. faecium C1(KF2) were highly susceptible to bile salts, while P. pentosaceus VNK-1 (KK4) and P. pentosaceus H11 (KF3) showed the highest resistance.”

compound_effect100% confidence

Pediococcus pentosaceus VNK-1 (KK4) higher antimicrobial activity

“The antimicrobial activity of P. pentosaceus VNK-1 (KK4) and P. pentosaceus H11 (KF3) was higher than other isolates and consequently, their inhibition zones were larger.”

compound_effect100% confidence

Pediococcus pentosaceus H11 (KF3) higher antimicrobial activity

“The antimicrobial activity of P. pentosaceus VNK-1 (KK4) and P. pentosaceus H11 (KF3) was higher than other isolates and consequently, their inhibition zones were larger.”

compound_effect100% confidence

Lactiplantibacillus plantarum PM411 (KK5) decreased 9 Log CFU/mL

“Only the numbers of L. lactis RSg (KK1) and Lpb. Plantarum PM411 (KK5) decreased to below 9 Log CFU/mL after acidic conditions (pH = 3) and showed weak antibacterial activity.”

compound_effect100% confidence

Enterococcus faecium C1 (KF2) susceptible bile salts

“Enterococcus mundtii CECT972T (KK3) and E. faecium C1(KF2) were highly susceptible to bile salts, while P. pentosaceus VNK-1 (KK4) and P. pentosaceus H11 (KF3) showed the highest resistance.”

compound_effect100% confidence

all isolates resistant tetracycline

“All of the isolates were resistant to tetracycline and sensitive to chloramphenicol and gentamicin.”

compound_effect100% confidence

all isolates sensitive gentamicin

“All of the isolates were resistant to tetracycline and sensitive to chloramphenicol and gentamicin.”

compound_effect100% confidence

Lactococcus lactis RSg (KK1) decreased 9 Log CFU/mL

“Only the numbers of L. lactis RSg (KK1) and Lpb. Plantarum PM411 (KK5) decreased to below 9 Log CFU/mL after acidic conditions (pH = 3) and showed weak antibacterial activity.”

compound_effect100% confidence

Pediococcus pentosaceus VNK-1 (KK4) resistant bile salts

“Enterococcus mundtii CECT972T (KK3) and E. faecium C1(KF2) were highly susceptible to bile salts, while P. pentosaceus VNK-1 (KK4) and P. pentosaceus H11 (KF3) showed the highest resistance.”

compound_effect100% confidence

Enterococcus mundtii CECT972T (KK3) susceptible bile salts

“Enterococcus mundtii CECT972T (KK3) and E. faecium C1(KF2) were highly susceptible to bile salts, while P. pentosaceus VNK-1 (KK4) and P. pentosaceus H11 (KF3) showed the highest resistance.”

compound_effect100% confidence

all isolates sensitive chloramphenicol

“All of the isolates were resistant to tetracycline and sensitive to chloramphenicol and gentamicin.”