Assessing the Safety and Probiotic Characteristics of Lacticaseibacillus rhamnosus X253 via Complete Genome and Phenotype Analysis
Lei Zhao, Yinan Zhang, Yang Liu, Jiang Zhong, Dong Zhang
Microorganisms
Abstract
<i>Lacticaseibacillus rhamnosus</i> is a generalist that can adapt to different ecological niches, serving as a valuable source of probiotics. The genome of <i>L. rhamnosus</i> X253 contains one chromosome and no plasmids, with a size of 2.99 Mb. Both single-copy orthologous gene-based phylogenetic analysis and average nucleotide identity indicated that dairy-derived <i>L. rhamnosus</i> X253 was most closely related to the human-intestine-derived strain <i>L. rhamnosus</i> LOCK908, rather than other dairy strains. The adaptation of <i>L. rhamnosus</i> X253 and the human-intestine-derived strain <i>L. rhamnosus</i> GG to different ecological niches was explained by structural variation analysis and COG annotation. Hemolytic assays, API ZYM assays, and antimicrobial susceptibility tests were performed to validate risk-related sequences such as virulence factors, toxin-encoding genes, and antibiotic-resistance genes in the genomes of <i>L. rhamnosus</i> X253 and GG. The results showed that <i>L. rhamnosus</i> GG was able to use L-fucose, had a higher tolerance to bile salt, and adhered better to CaCo-2 cells. In contrast, <i>L. rhamnosus</i> X253 was capable of utilizing D-lactose, withstood larger quantities of hydrogen peroxide, and possessed excellent antioxidant properties. This study confirmed the safety and probiotic properties of <i>L. rhamnosus</i> X253 via complete genome and phenotype analysis, suggesting its potential as a probiotic.
Extracted Claims
3 claims extracted from this paper into the knowledge graph
Lacticaseibacillus rhamnosus X253 possessed excellent antioxidant properties
“In contrast, <i>L. rhamnosus</i> X253 was capable of utilizing D-lactose, withstood larger quantities of hydrogen peroxide, and possessed excellent antioxidant properties.”
Lacticaseibacillus rhamnosus X253 was capable of utilizing D-lactose
“In contrast, <i>L. rhamnosus</i> X253 was capable of utilizing D-lactose, withstood larger quantities of hydrogen peroxide, and possessed excellent antioxidant properties.”
Lacticaseibacillus rhamnosus X253 withstood larger quantities of hydrogen peroxide
“In contrast, <i>L. rhamnosus</i> X253 was capable of utilizing D-lactose, withstood larger quantities of hydrogen peroxide, and possessed excellent antioxidant properties.”