Effects of CaCl2 concentration on fibrils formation and characteristics of soybean protein isolate and β-conglycinin/glycinin
Liming Miao, Jianyu Zhu, Xinhui Peng, Jianling Feng, Hongxia Dong, Xiaohong Tong +2 more
Food Hydrocolloids
Abstract
In this study, fibrillation of soybean protein isolate (SPI) and β-conglycinin (7S)/glycinin (11S) in acid-heating with CaCl 2 concentration (0–200 mM) was explored. Fibril formation kinetics results showed the fastest fibril formation rate and the most fibrils with 80 mM CaCl 2 addition. Fourier-transform infrared (FTIR) spectroscopy, size distribution and Transmission electron microscopy (TEM) results demonstrated SPI fibril structure became more complete and occurred entanglement with CaCl 2 concentration increasing from 0 to 80 mM. When CaCl 2 addition was above 80 mM, ζ-potential, surface hydrophobicity ( H 0 ) and free sulphydryl groups determination proved hydrophobic interaction among fibril built-units was hindered by charges shielded effect, and basic units of 11S were more exposed, aggregate morphology were changed to thicker fibrils or amorphous aggregates. The whole system with CaCl 2 has preferable antioxidant property and no cytotoxicity. The study provided a basis for optimizing SPI fibrillation in food industry. • Soybean protein isolate (SPI) fibril formation was improved in certain CaCl 2 concentration (80 mM). • Glycinin (11S) takes main responsibility in SPI fibrils transition affected by ionic strength. • Fibril system with CaCl 2 addition results in excellent biological activity.
Extracted Claims
4 claims extracted from this paper into the knowledge graph
80 mM CaCl2 improves SPI fibril formation
“Fibril formation kinetics results showed the fastest fibril formation rate and the most fibrils with 80 mM CaCl2 addition.”
CaCl2 concentration above 80 mM hinders hydrophobic interaction among fibril built-units
“When CaCl2 addition was above 80 mM, ζ-potential, surface hydrophobicity (H0) and free sulphydryl groups determination proved hydrophobic interaction among fibril built-units was hindered by charges s...”
CaCl2 concentration affects SPI fibril structure
“Fourier-transform infrared (FTIR) spectroscopy, size distribution and Transmission electron microscopy (TEM) results demonstrated SPI fibril structure became more complete and occurred entanglement wi...”