Leafy Vegetable Nitrite and Nitrate Content: Potential Health Effects

Abstract

The aim of this research was to determine the concentrations of nitrates and nitrites in different types of vegetables that are commonly represented in the diet of the inhabitants of Split and Dalmatian County. Therefore, using the method of random selection, there were 96 samples of different vegetables. The determination of the nitrate and nitrite concentrations was carried out by high-pressure liquid chromatography (HPLC) with a diode array detector (DAD). The nitrate concentrations in the range 2.1-4526.3 mg kg^-1 were found in 92.7% of the analyzed samples. The highest nitrate values were found in rucola (<i>Eruca sativa</i> L.) followed by Swiss chard (<i>Beta vulgaris</i> L.). In 36.5% of the leafy vegetables intended for consumption without prior heat treatment, nitrite was found in the range of 3.3-537.9 mg kg^-1. The high levels of nitrite in the vegetables intended for fresh consumption and the high nitrate values in Swiss chard indicate the need to establish maximum nitrite limits in vegetables, as well as the broadening of legal nitrate limits to wide varieties of vegetables.