Extraction optimization and structural characterization of soluble dietary fiber in foxtail millet and its inhibitory activities on colon cancer
Aiqi Ren, Liying Chen, Wenjing Zhao, Shuhua Shan, Zhuoyu Li, Zhaohui Tang
Journal of Functional Foods
Abstract
Soluble dietary fiber has anti-tumor effect and is closely related to human health. At present the structure of soluble dietary fiber from foxtail millet (FMB-SDF) and its anti-tumor effect remain unclear. In this study, the structure of FMB-SDF was characterized, and its effects on the colon cancer were investigated. The results showed that under optimal extraction conditions the yield of FMB-SDF was 14.25 %. Based on the monosaccharide composition, methylation results and NMR analysis, the preliminary structure of FMB-SDF was inferred to be a glucan. Interestingly, FMB-SDF markedly inhibited the colony formation ability of HCT116 and HT-29 cells and could significantly induce the increase of reactive oxygen species (ROS) and apoptosis of HCT116 and HT-29 cells. The results of animal experiments showed that FMB-SDF could significantly alleviate clinical symptoms of colon cancer in mice. These results provide a theoretical basis for the exploitation and utilization of FMB-SDF against colon cancer.
Extracted Claims
4 claims extracted from this paper into the knowledge graph
FMB-SDF (Soluble Dietary Fiber from Foxtail Millet) is a glucan
“Based on the monosaccharide composition, methylation results and NMR analysis, the preliminary structure of FMB-SDF was inferred to be a glucan.”
extraction of FMB-SDF yields 14.25%
“The results showed that under optimal extraction conditions the yield of FMB-SDF was 14.25 %.”
FMB-SDF (Soluble Dietary Fiber from Foxtail Millet) alleviates clinical symptoms of colon cancer in mice
“The results of animal experiments showed that FMB-SDF could significantly alleviate clinical symptoms of colon cancer in mice.”