Resuscitation of viable but nonculturable bacteria promoted by ATP-mediated NAD+ synthesis

Abstract

Our findings revealed a strategy employed by VBNC cells for revival, that is, using residual ATP to primarily recover metabolic activity, driving cells to exit dormancy. The synthesis pathway of lipopolysaccharide (LPS) in rfaL null mutant was inhibited and could supply more ATP to synthesis NAD⁺ and promote resuscitation.