Separation of trichothecene mycotoxins in a matrix of hemp ( <i>Cannabis sativa</i> L.) seed, and quantification of deoxynivalenol in hemp grain infected with <i>Fusarium graminearum</i>
Isabelle A. Kagan, Magdalena Ricciardi, Henry Smith, Rachel R. Schendel, Robert Pearce, Nicole Gauthier
JSFA reports
Abstract
Abstract Background Fusarium graminearum , one of the causal agents of ear rot in maize and head blight of cereal grains, also infects hemp ( Cannabis sativa L. containing a maximum of 0.3% tetrahydrocannabinol or THC). This fungal pathogen produces trichothecene mycotoxins when it infects maize and cereal grains, but little is known about its production of mycotoxins in hemp. A method was developed to separate mycotoxins deoxynivalenol (DON), 15‐acetyldeoxynivalenol (15‐ADON), and 3‐acetyldeoxynivalenol (3‐ADON) in commercial hemp seed and grain (harvested tissue), using high‐performance liquid chromatography (HPLC) with ultraviolet detection. Results In HPLC analysis of commercial hemp seed, DON was separated from matrix components. Distortion of the DON peak at higher concentrations led