Artificial Sweeteners in US-Marketed Oral Nicotine Pouch Products: Correlation With Nicotine Contents and Effects on Product Preference.

Abstract

Artificial sweeteners, sucralose and acesulfame-k, are listed as ingredients of oral nicotine pouches (ONPs), a product category with a rapidly growing market share. The exact quantities of these sweeteners in ONPs remain unknown. Artificial sweeteners in ONPs may reduce aversion, facilitate initiation, and encourage consumption behavior. Sucralose and acesulfame-k contents in major US-marketed ONP brands (Zyn, on!, and Velo) were determined by liquid chromatography-mass spectrometry. Sweetener effects on aversion and consumption of ONPs were modeled in single- and two-bottle drinking tests, offering mice ONP extracts calibrated to contain nicotine levels similar to the saliva of people who use smokeless tobacco. To examine the contribution of sweet taste perception, consumption behavior was compared between wild-type and sweet taste receptor-deficient mice (Tas1r2-/-). Acesulfame-k was detected in on!, Zyn, and Velo ONPs (~0.3 to 0.9 mg/pouch), including products marketed as "Unflavored" or "Flavor ban-approved." In Velo ONPs, sweetened with sucralose (~0.6 to 1.2 mg/pouch), higher nicotine strength products contained higher sucralose levels. Tas1r2-/- mice consumed less ONP extracts than wild-type mice in both sexes. ONP extracts with both higher nicotine and sweetener strengths were tolerated by wild-type mice, but produced stronger aversion in Tas1r2-/- mice. ONPs contain significant amounts of artificial sweeteners acesulfame-k and sucralose, with some brands adding more sweeteners to higher nicotine strength ONPs. In mice, artificial sweeteners, at levels present in ONPs, increase nicotine consumption. Increasing sweetener contents facilitates the consumption of higher nicotine strength ONPs. Sweetness imparted by sweetener addition to ONPs likely reduces the aversive sensory effects of nicotine and other ONP constituents. Artificial sweeteners such as acesulfame-k or sucralose reduce aversion and likely facilitate the consumption of ONPs. The market