Weak phospholipase-positive Listeria monocytogenes: a challenge for the diagnostic laboratory.
Marc J A Stevens, Joseph Wambui, Sabrina Corti, Magdalena Nüesch-Inderbinen, Roger Stephan
Diagnostic microbiology and infectious disease
Abstract
Listeria monocytogenes isolates from environmental samples of a food producing company harboured a C424G mutation in the prfA gene that results in a G141A substitution in PrfA. This mutation results in strongly decreased phospholipase activity and is therefore challenging for culture-based detection of L. monocytogenes and may even lead to misidentification.