Vancomycin-bacterial imprinted polymer hybrid for viable Staphylococcus aureus highly efficient capture, photothermal inactivation, and sensitive detection.

Abstract

To facilitate the capture, inactivation, and detection of viable Staphylococcus aureus (SA) in food, a vancomycin (Van)-bacterial imprinted polymers (BIPs) hybrid receptor was fabricated by introducing Van into SA-imprinted polydopamine (PDA) via oriented surface imprinting. Taking advantage of the dual recognition arising from Van's affinity for peptidoglycan and BIPs' imprinting effect on SA, the Van-BIPs hybrid exhibited better capture performance than Van or BIPs alone. Leveraging the photothermal effect of PDA, the captured SA could be in situ lysed within 5 min under near-infrared irradiation, causing the release of adenosine triphosphate (ATP) from SA. Using ATP as biomarker, as low as 13.7 CFU/mL of viable SA was able to be detected within 38 min by integrating Van-BIPs hybrid with ATP-bioluminescence assay. Spiked food samples were also successfully analyzed with the recoveries of 85.71 %-106.11 %. The Van-BIPs hybrid might offer a promising tool to control SA in food industries.