A novel fluorescent aptasensor using magnetic silica nanospheres and Ag@MOF nanocomposites for extraction and detection of Salmonella Typhimurium in eggs.

Abstract

Salmonella Typhimurium (S. Typhimurium) is one of the most common food-borne diseases, highlighted as the top food-borne bacterial pathogen in the world with a low infectious dose (1 CFU) and high mortality rate. It is commonly associated with numerous foods such as dairy products, protein sources (multiple types of meat, poultry, and eggs), and bakery products. Therefore, an accurate and rapid detection method for this bacterium is always needed. This study used a fluorescent platform based on extraction and detection of the S. Typhimurium in eggs. Specifically, S. Typhimurium was isolated using aptamer-functionalized magnetic nanoparticles and then detected using a fluorescent method by aptamer-functionalized metal-organic frameworks. Under optimal conditions, this study achieved a linear detection range of 7.5 to 7.5 × 10⁶ CFU/mL, a detection limit of 4.61 CFU/mL, and a total detection time of 90 min. The recovery range for egg samples was 97.0-101.0%, proposing high accuracy of the developed probe in real samples. Also, the specificity study results confirmed excellent selectivity of the probe in the presence of non-target bacteria, arising from the stable interactions between the S. Typhimurium and aptamer-functionalized probes. This platform serves as an effective in situ probe for fast and reliable control of eggs and may also be validated for other food samples.