Residue and dietary risk assessment of 8.6% emamectin benzoate and tetraniliprole suspension concentrate in rice.

Abstract

Emamectin benzoate and tetraniliprole are effective insecticides against Cnaphalocrocis medinalis on rice. However, there are few studies on the residue analysis method for the simultaneous detection of emamectin benzoate and tetraniliprole and their metabolites BCS-CQ63359 on rice. Here, QuEChERS (quick, easy, cheap, effective, rugged, and safe extraction method) combined with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was used to establish an analytical method for the simultaneous detection of emamectin benzoate, tetraniliprole and BCS-CQ63359 in brown rice, rice husk and straw, and to explore the residual degradation dynamics and dietary risk assessment. The results demonstrated that the recoveries of emamectin benzoate B1a, tetraniliprole, and BCS-CQ63359 ranged from 84% to 99%, with a relative standard deviation of 0.86% to 8.6%. The lowest limit of quantification (LOQ) for all compounds was 0.02 mg kg-1. Additionally, field trials conducted at 12 locations revealed that the final residues of emamectin benzoate and tetraniliprole in both rice and brown rice remained within acceptable limits. Dietary risk assessments indicated that the daily intakes of emamectin benzoate and tetraniliprole were 0.0264 mg and 0.161 mg, respectively, posing no significant health risks to the general population. The optimization of a method for determining emamectin benzoate B1a, tetraniliprole, and its metabolite BCS-CQ63359 residues in rice using QuEChERS pretreatment and UPLC-MS/MS detection has been achieved. The linear range, correlation coefficient, accuracy, precision, and LOQ all satisfied the testing standards for pesticide residues in crops. The results indicate that the exposure risks associated with emamectin benzoate and tetraniliprole are deemed acceptable. The dietary risk assessment reveals that the dietary risk probabilities for both emamectin benzoate and tetraniliprole are below 1, falling within the