[Determination of 6 kinds of biogenic amines in livestock, poultry and aquatic prefabricated food by ultra-performance liquid chromatography-triple quadrupole mass spectrometry].

Abstract

To establish a non-derivatization detection method based on solid-phase extraction-ultra performance liquid chromatography-triple quadrupole mass spectrometry for the simultaneous and quantitative determination of cadaverine, putrescine, histamine, tyramine, tryptamine and phenethylamine in livestock, poultry and aquatic prefabricated food. Samples were extracted with 5% aqueous perchloric acid-acetonitrile(2∶8, V/V), and purified with Oasis PRiME HLB solid-phase extraction column. The target compounds were separated on ZORBAX RRHD Eclipse Plus C18 column(3.0 mm×150 mm, 1.8 μm) by gradient elution using 0.1% formic acid aqueous solution and methanol as mobile phase. The detection was performed with electrospray ionization(ESI) in positive mode under multiple reaction monitoring(MRM) mode, and quantified by internal standard method. Six target compounds of cadaverine, putrescine, histamine, tyramine, tryptamine and phenethylamine were well reserved on the chromatographic column, and the linear range was 10-2000 μg/L(R~2>0.999). The limit of detection and limit of quantitation were 5 μg/kg and 10 μg/kg, respectively. The recovery rates of the six compounds ranged from 71.6% to 105.2%, and the relative standard deviations were 0.73% to 12.64%(n=6). A total of 103 livestock, poultry and aquatic prefabricated food were tested for the target compounds. The detection rates of putrescine, tyramine, tryptamine, cadaverine, and phenethylamine were 96.1%, 60.2%, 22.3%, 34.0%, and 58.2%, respectively. This method has the advantages of simple pretreatment, fast analysis speed, high accuracy and sensitivity, which is suitable for the determination of six biogenic amines in livestock, poultry, and aquatic prefabricated food.